About the mechanism of interference of silver staining with peptide mass spectrometry
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چکیده
منابع مشابه
About the mechanism of interference of silver staining with peptide mass spectrometry.
The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demonstrated that this interference increases with time between silver staining and gel processing, although the silver image is constant. This suggested an important role of the formaldehyde used in silver staining develop...
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15 صفحه اولSweet silver: a formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry.
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features, but its compatibility with MS is limited. We describe here, a new variant of silver staining that is completely formaldehyde-free. Reducing su...
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ژورنال
عنوان ژورنال: PROTEOMICS
سال: 2004
ISSN: 1615-9853,1615-9861
DOI: 10.1002/pmic.200300642